In this podcast, you will learn how you can:
• Go beyond cryo-confocal: Use spectral signatures to unlock new layers of cellular information.
• Correlate across scales: Learn how to integrate cryo light and electron imaging for richer insights.
• Bridge modalities: Explore how X-ray tomography connects to cryo-EM analysis.

Watch the full presentation here: https://microscopyfocus.com/see-the-hidden-advancing-cryo-workflows-for-cellular-discovery/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this podcast, you will:
• Understand the challenges of traditional automated systems in 3D cell culture quantification
• Gain insights into the benefits of non-consumable, slide-free cell counting technology
• Learn how to implement user-defined parameters for customized cell counting protocols
• Discover how DeNovix CellDrop Automated Cell Counter uses machine learning for accurate organoid, tumor spheroid, and spheroid counting

Watch the full presentation here: https://events.bitesizebio.com/learn-to-automate-organoid-counting

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this podcast, you will learn:
• When to choose screening assays versus qualified or verified assays to effectively meet your research objectives
• Key techniques that contribute to the reliability and consistency of immunoassays
• How to leverage analytical tools to assess immunoassay results for improved experimental outcomes

Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-boost-assay

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this podcast, you will learn:

• How to streamline serial sectioning for volume EM using the UC Enuity and AT module;
• How to achieve in-situ targeting under cryogenic conditions for cryo-EM workflows;
• How integrated imaging and AI-powered analysis enhance 3D EM data interpretation.

Watch the full presentation here: https://microscopyfocus.com/integrated-solutions-for-serial-sectioning-and-cryo-em-with-uc-enuity/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this webinar, you will learn:
• A groundbreaking tissue-clearing technique for precise whole-brain 3D spatial RNA imaging at single-cell resolution;
• How to use it for robust 3D RNA imaging of whole organs of various sizes from different species;
• How to uniformly stain whole organs while enhancing tissue transparency and preserving RNA integrity.

Watch the full presentation here: https://events.bitesizebio.com/whole-brain-spatial-transcriptomics

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, you’ll discover how ELISpot and FluoroSpot assays overcome these challenges. These cell-based immunoassays measure cytokine and antibody secretion at the single-cell level, offering a sensitive, robust, and accessible way to capture rare immune responses.

You’ll learn how ELISpot and FluoroSpot can be applied in vaccine studies, biotherapeutic evaluation, and cell and gene therapy development. The episode also explores practical guidance on assay setup and execution, the advantages of multiplex cytokine detection with FluoroSpot, and how these platforms can be qualified for use in preclinical and clinical trials.

With their unique combination of sensitivity and ease of use, ELISpot and FluoroSpot provide a valuable alternative to flow cytometry, especially when high throughput and efficient data collection are priorities.

Watch the full presentation here: https://events.bitesizebio.com/detecting-rare-t-cell-responses-at

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, explore the root causes of waste in our industry, as we draw on real-world examples of failed companies, discontinued pipelines, and canceled orders. We will examine the ripple effects this has on both operational efficiency and the broader sustainability goals of life science organizations.

We will also introduce Wasteless Bio, a dedicated marketplace designed to keep high-quality, unused products in circulation—helping researchers access discounted reagents and instruments while reducing environmental impact.

Whether you are a biotech executive, lab manager, or supplier, you will gain a clearer understanding of how to rescue valuable resources from the trash and reinvest them back into scientific innovation.

Watch the full presentation here:

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

However, with the latest developments in cell-counting hardware and app technology, automated counting of even challenging cells is simpler than ever.

In this episode, see how you can achieve reliable automated cell counts with the CellDrop Automated Cell Counter.

Using hepatocytes as an example, you will learn how the machine learning technology integrated into DeNovix’s Hepatocyte app can save time, reduce human error, and provide consistent results.

Watch the full presentation here: https://events.bitesizebio.com/automated-hepatocyte-counting-new

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Mucins are a diverse family of high molecular weight glycoproteins that play critical roles in protecting and lubricating epithelial surfaces, making them integral to numerous physiological processes and disease states.

As a promising class of biomarkers, mucins may offer unique insights into various pathologies, such as cancer and chronic inflammatory diseases. However, their complex structures and extensive glycosylation present significant challenges in their study and application.

Mucin-domain glycoproteins are densely O-glycosylated and play key roles in many biological functions.

However, their dense O-glycosylation remains enigmatic both in the glycoproteomic landscape and structural dynamics, primarily due to the challenges associated with studying mucin domains. 

In this episode, you will get an introduction to the (glyco)proteomic techniques used in Mucinomics and learn how proper sample preparation of mucin-containing tissues for mass spectrometry is key to discovery.

MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.

Watch the full presentation here:

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Hear from real user experience how the advanced xMAP INTELLIFLEX® DR-SE Instrument revolutionizes assay multiplexing, enabling simultaneous rapid data acquisition for multiple sample parameters to expand the depth and breadth of your research.

Watch the full presentation here: https://events.bitesizebio.com/user-experience-of-the-xmap

This compact, flow-based multiplex platform integrates xMAP® Technology with modern enhancements to streamline assay development, enhance user experience, and deliver fast, reliable results by acquiring data for two parameters per analyte simultaneously.

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

The key reagents included in an immunoassay can significantly impact reliability and reproducibility, underscoring the importance of understanding critical components within immunoassay kits.

When selecting an immunoassay platform, it's essential to consider critical elements such as multiplexing capabilities and sensitivity needs as part of the experimental design.

Additionally, understanding the various assay formats and their data outputs can aid in the immunoassay selection process.

In this episode, immunoassay experts give practical guidance in these areas, empowering researchers to achieve more precise and meaningful results, thereby advancing knowledge and understanding in various scientific fields.

MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not For Use In Diagnostic Procedures.

Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-strategies-to

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This episode of Listen In explores the incorporation of AIVIA, an AI-powered image analysis software, into workflows to address these challenges in analyzing multicolor fluorescent microscopy data of 3D organoids.

See how AIVIA’s tools enhance the visualization and quantification of cellular structures and organoids, enabling precise segmentation and analysis of fluorescent signals.

And get a showcase of how AIVIA supports Python-based image analysis for microscopists.

Watch the full presentation here: https://events.bitesizebio.com/ai-powered-3d-organoid-analysis

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations.

Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method.

Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate.

Larger batches would require multiple analysts for a single-day turnaround time.

With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential.

Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements.

It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL.

Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/join

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From modest experiments to expansive 96-plex studies and beyond, MULTI-seq enables truly mechanistic studies. Hit play to explore the potential of this tool and how it could benefit your research.

Watch the full presentation here: https://events.bitesizebio.com/multiplexing-magic-how-multi-seq/join

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Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy.

This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics.

In this episode of Listen In, hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity.

See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.

We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration.

You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems.

Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/

"Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

See how to characterize tissue microenvironments and examine the differences between normal and disease tissues without the need to code or train Deep Learning models—all with Aivia.   

Plus, get your 3D spatial biology results faster with an inbuilt, enhanced Deep Learning model that can accurately detect and partition cells with morphological variations.

You will discover how to leverage your expertise or simple automation to classify cells into different phenotypes and interactively explore them in their spatial context. 

We also demonstrate how, with a few clicks, you can produce key measurements such as percentage distribution, Pearson correlation coefficient, and dimensionality reduction. You will also learn how to visualize the relationship between biomarkers and clusters using dendrograms.

Watch the full presentation here: https://microscopyfocus.com/enhancing-3d-spatial-biology-with-ai-simplified-insights-for-all/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

They participate in creating, developing, and disseminating guidelines, resources, and tools to improve image-based research.

In this episode, learn what impacts reproducibility in microscopy, discover resources and initiatives for improving education, rigor, and reproducibility, and see how collaboration drives innovation.

Watch the full presentation here: https://microscopyfocus.com/accelerate-scientific-progress-the-power-of-reproducibility-collaboration-and-new-imaging-technologies/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Sample selection and preparation are of utmost importance, as they have a direct impact on the accuracy, reliability, and integrity of the obtained results.

Properly selecting and preparing samples ensures that they are representative of the population under study and that the analytes of interest are preserved in their native state.

In addition, controlling and mitigating matrix interference is crucial in immunoassays to minimize the influence of sample components that may hinder the accuracy of measurements.

In this episode, hear from immunoassay experts as they guide you through all these areas.
 
Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-best-practices/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

But pathological events such as ischemia (stroke), trauma (traumatic brain injury), or tumor growth (gliomas and brain metastasis), activate the vascular endothelium, disturb or disrupt the BBB and allow immune cell homing.

In this episode, discover how innate immunity can sustain deleterious effects following brain trauma, and the technological developments enabling longitudinal studies into post-trauma lesion remodeling.

Watch the full presentation here: https://microscopyfocus.com/windows-on-neurovascular-pathologies/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode, explore how combining laser microdissection with mass spectrometry (LCM-MS) on fixed and stained tissues lets you explore the protein networks within pathological features to shed light on their molecular functions.

Using hallmark lesions of lung fibrosis in the honeycomb airways and fibroblastic foci as an example, you'll hear how this technology can detect over 4,900 proteins from tissue volumes as low as 0.005 mm3.

Watch the full presentation here: https://events.bitesizebio.com/unleashing-fibrotic-secrets/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously.

Watch the full presentation here: https://microscopyfocus.com/confocal-microscopy-stellaris-showcase/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Antibodies intended for use as antibody-drug conjugates (ADCs) should specifically bind to target cells and rapidly internalize via endocytosis.
However, the ability to study these processes has historically been limited by the lack of tools that directly monitor the internalization and subsequent catabolism of extracellular material.

In this episode, get a demo of rapid, high-throughput methods to screen for antibody internalization. You will also hear about techniques to directly visualize the internalization of therapeutic antibodies and ADCs in live cells for structure-activity relationship studies.

You will also learn how the insights provided by these approaches have the potential to accelerate biotherapeutic lead generation and development.

Watch the full presentation here: https://events.bitesizebio.com/tools-techniques-for-the-study-of/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with the MANTIS system.

Learn how this innovative approach allows for the acquisition of up to 10 colors, providing detailed immune phenotyping of skin samples. Plus, discover how MANTIS simplifies complex imaging, making advanced analysis accessible and practical for routine clinical practice.

Tune in to explore how this new technology can revolutionize the identification of skin immune cells and enhance diagnostic accuracy.

Watch the full presentation here: https://microscopyfocus.com/multiplex-annotated-tissue-imaging/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode, we're joined by a panel of individuals who have carved out unique careers that merge science with creativity. Our panelists include a Marketing Communication Manager/Graphic Designer, an Artist in Residence, and a Science Rapper.

These trailblazers will share their journeys, discuss how they found their unique paths, and provide insights into their innovative careers.

Learn about the various opportunities available beyond the conventional academic and industry roles, and get inspired by the possibilities of combining science with art.

Tune in to discover how you can turn your passion for science and art into a fulfilling career.

Watch the full presentation here: https://events.bitesizebio.com/breaking-out-of-the-mold-a-panel-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching.

Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels.

Discover how researchers successfully depleted five fluorochromes across different channels using a single laser and utilized the phasor plot for quick and easy visualization of staining quality and lifetime analysis. This approach not only doubles the number of distinguishable colors in laser scanning microscopy but also simplifies the process, making it accessible to many scientists with minimal training.

Watch the full presentation here: https://microscopyfocus.com/five-color-sted/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Learn how to tackle issues like:

• Career disruption
• Reason for choosing your place of work
• Track record relative to opportunity
• Consumer involvement

This episode guides you through the rationale behind these additional requirements, offers tips on how to effectively showcase your track record and collaborators, and provides insights on managing challenging grant situations. Don’t miss this essential advice that could make all the difference in securing your next grant!

Watch the full presentation here: https://events.bitesizebio.com/grant-writing-everything-but-the-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com

But now, scientists are breaking these barriers with fluorescence lifetime multiplexing. This innovative approach uses differences in fluorescence lifetimes to distinguish between similar dyes, allowing for more complex imaging.

Hear how researchers combined lifetime multiplexing with spectral detection to analyze multiple targets simultaneously. Discover the exciting advancements made by characterizing 18 synthetic fluorophores and developing unique self-labeling protein tags.

These breakthroughs enable the imaging of up to eight targets in four spectral channels, providing unprecedented detail in cellular studies.

Watch the full presentation here: https://microscopyfocus.com/live-cell-fluorescence-lifetime-multiplexing-using-organic-fluorophores/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, we explore the various stresses that scientists encounter in their daily lab life and discuss effective strategies to manage them. Learn how to prevent burnout, anxiety, and depression by addressing issues like perfectionism and impostor syndrome. Our expert shares personal insights and practical advice on cultivating a resilient and healthier growth mindset.

Hit play to discover that you're not alone in feeling overwhelmed and that with the right mindset, you can navigate the pressures of a scientific career more effectively.

Watch the full presentation here: https://events.bitesizebio.com/stress-in-the-lab-common-pitfalls-1/join

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Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions.

Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.

Join Dr. Padilla-Parra in this episode of Listen In to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.

Watch the full presentation here: https://microscopyfocus.com/visualizing-protein-protein-interactions-by-non-fitting-and-easy-fret-flim-approaches/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com

Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing.

In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and visualizing debris in a sample.

You will also get tips to optimize your existing nuclei extraction protocols from an experienced Application Scientist.

Watch the full presentation here: https://events.bitesizebio.com/optimizing-nuclei-extraction/join

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She describes the impact of research communication in and out of the lab and provides some guides to explaining your research in various contexts, such as lab tours, community visits, and other public engagements.

As well as learning how to work out your h-index, you will learn how to describe the impact of your papers.

Watch the full presentation here: https://events.bitesizebio.com/how-to-effectively-communicate-your-1/join

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Because of their diverse physiological and pathophysiological roles, GPCRs are also major drug targets.

Our understanding of GPCR signaling has evolved from single receptors at the cell surface activating specific heterotrimeric G protein pathways, to an increasingly complex receptor signaling system.  However, the complexity of GPCR signaling is translated to specific cellular and physiological responses is still unclear.

In this episode, we explore some of the latest endeavors to uncover GPCR signaling mechanisms through a wide range of imaging applications, from analyzing single molecules to the identification of new subcellular signal platforms.

Watch the full presentation here: https://microscopyfocus.com/imaging-gpcr-activity-unravelling-mechanisms-from-molecules-to-morphology/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Manuscript reviewing requires specific skills and knowledge to properly assess published literature. By reading and reviewing papers, researchers can develop and hone these skills, which will help them identify and handle tricky issues such as image and data manipulation.

In this episode of Listen In, Dr. Johanna Ahlskog discusses the essential skills all reviewers and researchers need, emphasizes the critical importance of the review process, and offers general considerations when reviewing articles.

Watch the full presentation here: https://events.bitesizebio.com/critical-analysis-of-research-1/join

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Confocal imaging is a fundamental tool for studying the complex interplay of biomolecules, molecular machines, and higher-order cellular structures owing to its optical sectioning, sensitivity, and temporal and spatial resolution capabilities.

Imaging complex cellular structures at nanoscale resolution while characterizing the dynamics of multiple species in live specimens are emerging avenues to shed light on biological processes in their native physiological context.

With the advent of STED, researchers have realized the visualization of intracellular structures at the nanoscale. Tune in to see how STED is enabling unprecedented insights into cellular behavior, interactions, and function.

Watch the full presentation here: https://microscopyfocus.com/new-tausted-tools-for-gentle-live-imaging-at-nanoscale-resolution/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, you'll learn to find the ideal hook for your science story.

This trick, borrowed from journalism, involves taking a step back and thinking about all the aspects of your research and where they might overlap with other topics that attract interest from other people.

Knowing how your research connects with other areas is useful when writing grant proposals or giving public talks about your work because it helps you write or speak persuasively.

Watch the full presentation here: https://events.bitesizebio.com/finding-the-ideal-hook-for-your/join

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This episode of Listen In gives you valuable insights into often overlooked areas of your filtration processes and will help you safeguard your sensitive cell cultures.

Explore the critical importance of sterile filtration in diverse applications leveraging Millipore® filtration technology.

Hear about the alarming consequences of suboptimal filters in sensitive media composition, highlighting the financial and time investments at stake.

Plus, discover how the use of Millipore® filters as the gold standard in both non-sterile and sterile filtration can mitigate contamination risks and uphold the integrity of your cultures.

Our expert guest showcases the superior performance of Millipore® membranes and explains their advanced features that enable efficient particle retention and exceptional flow characteristics. 

Watch the full presentation here: https://events.bitesizebio.com/culture-media-preparation/join

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A variety of automated liquid handling systems—suiting all budgets and workloads—are now available. These systems are designed to improve consistency and efficiency by eliminating both person-to-person and day-to-day variability.

But how to get started with automation? When does it make sense to automate workflows and methods, and isn't it super complicated to implement an automated liquid handler in a lab?

Get answers to these questions in this episode of Listen In!

Watch the full presentation here: https://events.bitesizebio.com/automate-your-pipetting-unleash/join

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Their research focuses on intrinsic and extrinsic mechanisms contributing to the formation of the brain by examining mutations in genes influencing cell-cell contacts, the extracellular matrix, and the secretion of vesicles.

In this episode of Listen In, Silvia presents the latest research that makes an important contribution to our understanding of cell non-autonomous mechanisms in the development of neurodevelopmental disorders.

She also explains how imaging workflows, using the Mica Imaging Microhub alongside confocal and super-resolution systems, support the group’s research.

Watch the full presentation here: https://microscopyfocus.com/cellular-crosstalk-in-neurodevelopmental-disorders/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Check out this enlightening discussion of the crucial factors that influence the outcome of PCR experiments and discover the parameters you can optimize for better and more consistent PCR results.

You will get reliable advice directly from PCR experts at Eppendorf on key topics, including the core features of thermal cyclers and the importance of good pipetting techniques to minimize evaporation.

This episode also features helpful insights into ideal reaction set-up, primer design, template DNA selection, and PCR optimization.

Watch the full presentation here: https://events.bitesizebio.com/end-point-pcr-set-up-and/join

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But how do we do it?

One way is to design, conduct, and report research that can maximize the impact of our individual and collective investment in research.

Plus, changing the research culture to prioritize 'getting it right' over 'getting it published' promotes transparency and reproducibility without losing the engine of innovation and discovery that drives scientific inquiry.

In this episode of Listen In, get an overview of the fundamental challenges that reduce the credibility and reproducibility of research, how open science practices address these challenges, how you can incorporate them into your research, and how you can help promote a change in the research culture.

Watch the full presentation here: https://events.bitesizebio.com/approaches-for-more-transparent/join

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However, results are not the objective of the scientific method, which involves proposing an answer to a question, designing experiments to determine whether the proposed answer is true or false, and objectively interpreting the results.

This episode of Listen In explores the definition of the scientific method and how to apply it to your research.

Get a handle on the scientific method with a clear definition, and learn how to apply it to your research using real-life examples of the scientific method in action.

You will come away from this tutorial with increased confidence in the rigor of your research and the fundamental knowledge to design scientifically sound experiments.

Watch the full presentation here: https://events.bitesizebio.com/confirmation-bias-and-the-1/join

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However, results are not the objective of the scientific method, which involves proposing an answer to a question, designing experiments to determine whether the proposed answer is true or false, and objectively interpreting the results.

Nonetheless, the results-focused attitude to doing science is widespread, and it comes with a huge drawback—it encourages confirmation bias—making us prone to searching for evidence and interpreting findings to support proposed answers we believe to be true.

This episode of Listen In explores what confirmation bias is and how to avoid it.

Learn why confirmation bias is so common, how it creeps into our thinking, and how it degrades the quality of our experimental designs and findings.

You will come away from this tutorial with the tools needed to defend against confirmation bias, challenge what you believe, and reorient yourself from a results-focused outlook to a method-focused one.

Watch the full presentation here: https://events.bitesizebio.com/confirmation-bias-and-the/

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In this episode of Listen In, see how the new Thermo Scientific Hydra Bio Plasma-Focused Ion Beam (PFIB) extends cryo technology to volume EM and enables the acquisition of high-resolution 3D data through automated serial milling and imaging of frozen-hydrated specimens.

Cryo Auto Slice & View enables the acquisition of high-resolution 3D data through automated serial milling and imaging for striking new insights into a range of frozen-hydrated specimens prepared by high-pressure freezing or plunge freezing.

The choice of four different plasma ion species offers advantages for imaging cryo- and resin-embedded samples. The Hydra Bio also features the Spin Mill Bio Method, an exclusive technique for large-area planar milling that yields similar-sized imaging areas as microtome slicing. Plus, see how Hydra Bio PFIB combines field-proven and new techniques to enable cryo and room temperature applications.

Watch the full presentation here: https://events.bitesizebio.com/cryo-tomography-to-volume-em/room

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The Principles of Humane Experimental Technique, the 3Rs (Replacement, Refinement, and Reduction), have been the mainstay of humane research involving animals for over fifty years.

However, it is becoming increasingly apparent that to enable high-quality ethical and humane science involving animals, other ethical constructs and issues, for example, research culture, reproducibility, reliability, and responsibility, must also be considered.

The 12Rs Framework brings these different constructs together in a single visual framework. Furthermore, it applies to all areas of scientific research, not just research involving animals.

Watch the full presentation here: https://events.bitesizebio.com/the-12rs-framework-enabling/join

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Generating insights into tissue microenvironments is crucial to our understanding of normal and abnormal tissue development, such as during cancer progression.

Spatial biology methods using microscopy and multiplexing techniques have allowed researchers to bring together protein expression profiles and spatial information to dive into the complex biology needed for these insights. However, image analysis of these datasets, especially with high numbers of protein markers and/or large numbers of cells, remains a challenge.

Artificial Intelligence (AI) image analysis has significantly progressed through innovations in deep learning, segmentation, and detection, making it a powerful tool for researchers.

Hear about the latest release of Aivia image analysis software, including AI tools, to help you tackle this challenge in your large 2D multiplexed image datasets.

Watch the full presentation here: https://microscopyfocus.com/elevate-your-research-with-spatial-insights/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

While glycoproteins are widely recognized as key molecular features involved in human health and disease, the analysis of protein glycosylation has historically been hampered by a lack of efficient and simple methods to characterize the analytically challenging glycan structures. Examples include structures exhibiting extensive heterogeneity and subtle isomeric features such as linkage and topological differences.

In this episode of Listen In, Dr. Thaysen-Andersen discusses opportunities and challenges in the burgeoning field of analytical glycoscience.

See the current landscape of glycobiology, learn about innovative analytical methods for N- and O-linked glycosylation profiling from biological specimens, and explore how advances in glycoanalytics enable new insights into human glycobiology.

MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.

Watch the full presentation here: https://events.bitesizebio.com/unleash-new-biological-insights/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, elevate your analysis and liquid chromatography. Learn what consumables, materials, and reagents can harm your LC-MS data for more accurate quantification using perfluoroalkyl substances (PFAS) as an example.

See how contamination from consumables, equipment, reagents, and non-specific binding of samples to syringe filters can make LC-MS quantification inaccurate.

Plus, explore the environmental impact of PFAS and how to choose syringe filters and other consumables for accurate quantification results.

Watch the full presentation here: https://events.bitesizebio.com/contamination-and-recovery-of-pfas/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nuclei, nucleoli, mitochondria, and lipid droplets.

Furthermore, analysis of the measured individual cell data can elucidate the temporal 3D volumetric dynamics with the dry mass information.

This episode presents the latest development of a low-coherence holotomography imaging system, HT-X1, and its numerous applications to different types of biological specimens, ranging from unicellular organisms to multicellular specimens.

Plus, learn how to combine holotomography with downstream molecular analysis, such as cell biology, immunology, microbiology, material science, and in vitro diagnosis.

Watch the full presentation here: https://events.bitesizebio.com/label-free-3d-live-cell-imaging-and/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Understanding why you need buffers, knowing how to choose the right one, and troubleshooting buffer issues are fundamental skills you can apply to make all your experiments work better—and you only need to learn how to prepare them correctly once.

In this episode, dive deep into the world of chemical buffers. Explore how to select the best buffer for your experiments, share practical tips on preparing buffer solutions, and troubleshoot any hiccups that might come your way. Plus, we'll demystify key concepts like pH and pKa.

Watch the full presentation here: https://events.bitesizebio.com/back-to-buffer-basics-everything/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, learn the nuts and bolts of scientific publication with a step-by-step tour of the whole process. Explore how to select the right journal and what tools are available to help make that decision. Plus, get advice on how to make the journal submission process go smoothly and how to take critical reviews and turn them into positives in your re-submission.

Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-iv-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

This is part four of our How To Write a Research Paper series:

I: Telling Your Story
II: The Anatomy
III: Common Problems And How To Overcome Them
IV: Understanding The Publication Process

In this episode of Listen In, we'll go through some of the structural reasons that scientific journals reject articles, how to solve common structural issues, and additional steps you can take to help your journal reviewers.

Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-iii-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

This is part three of our How To Write a Research Paper series:

I: Telling Your Story
II: The Anatomy
III: Common Problems And How To Overcome Them
IV: Understanding The Publication Process

In this episode of Listen In, we will delve deeper into each of these sections to show you how to tailor them to your audience.

Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-ii-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

This is part two of our How To Write a Research Paper series:

I: Telling Your Story
II: The Anatomy
III: Common Problems And How To Overcome Them
IV: Understanding The Publication Process

In this episode of Listen In, we will reframe the writing process to help you clearly and concisely communicate your research with great impact.

Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-i-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

This is part one of our How To Write a Research Paper series:

I: Telling Your Story
II: The Anatomy
III: Common Problems And How To Overcome Them
IV: Understanding The Publication Process

Plus, explore a knock-in mouse model of frontotemporal dementia that harbors a common mutation in the progranulin gene to test an antisense oligonucleotide (ASO)-based therapeutic strategy.

Watch the full presentation here: https://events.bitesizebio.com/using-antisense-to-characterize/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Neurons communicate at specialized junctions, or synapses, via chemical messengers called neurotransmitters. Within a millisecond of neuronal stimulation, synaptic vesicles packed with neurotransmitters fuse with the presynaptic plasma membrane and release their contents. These neurotransmitters then bind and activate receptors on the postsynaptic membrane, resulting in cell-to-cell signal transmission. Then, to sustain this neurotransmission, new vesicles are recruited to the release sites.

In this episode of Listen In, we dive into the powerful new technology to spatially and temporally investigate these dynamics.

Watch the full presentation here: https://events.bitesizebio.com/visualizing-membrane-dynamics-by/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode, learn how automated image analysis combined with flow cytometry can help improve data accuracy and precision, optimize and verify gating strategies, and discover novel biology, such as cell–cell interactions.

Join a panel of experts as they discuss the features, benefits, and applications of a paradigm-shifting technology in flow cytometry with the Invitrogen™ Attune™ CytPix™ Flow Cytometer.

Watch the full presentation here: https://events.bitesizebio.com/benefits-of-imaging-data-in-flow/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, explore the benefits of organoids as infectious disease models and drug screening vehicles, learn how to start organoid culture in your laboratory, and discover a portfolio of organoids for your research applications.

Watch the full presentation here: https://events.bitesizebio.com/jump-into-3d-organoids-basic/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/ 

This episode of Listen In with Cristy Gelling explores image manipulation. Many common types of image manipulation are classified by journals as scientific misconduct, even if you had no intent to deceive anyone.

Learn what practices are considered fraud or misconduct, how image formats and manipulations affect your data, and ways to process images that ensure your science is sound and your results are publishable.

Watch the full presentation here: https://events.bitesizebio.com/ethical-research-practice-3-image/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

This episode of Listen In with Karen O'Hanlon Cohrt explores publishing negative results and what is acceptable. Get answers to key questions, such as what is a negative result, and when should we report such results? What is the difference between a negative result and an unexpected result? What is an outlier? Plus, learn when it is okay to exclude an outlier and when you should include outliers to report the variation in your experiment.

Watch the full presentation here: https://events.bitesizebio.com/ethical-research-practice-ii-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

This episode of Listen In with Karen O'Hanlon Cohrt defines plagiarism and presents some relevant statistics on this topic. She also explores self-plagiarism, demonstrates some good online plagiarism checkers, and showcases some examples of plagiarism and ways to avoid it.

Despite the definition being straightforward, plagiarism is still a huge issue worldwide. It can be intentional and unintentional, which is why the key is to inform and educate researchers about what it entails.

Watch the full presentation here: https://events.bitesizebio.com/ethical-research-practice-i-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, discover the measures you can take to improve the reproducibility of your experiments by incorporating Good Cell Culture Practice into your eukaryote culture routine.

Explore the critical aspects of eukaryotic cell culture, including Mycoplasma test methods, effects of passage numbers, using Foetal Bovine Serum (FBS), and more!

Watch the full presentation here: https://events.bitesizebio.com/everyday-culture-practice-improving-3/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

• Reduced strain on thumbs and arms by improving pipetting ergonomics.
• Increased reproducibility of experiments with motor-operated pipetting cycles.
• Easy and efficient handling of high sample throughput.

In this episode of Listen In, learn why switching to electronic pipettes can be beneficial for a variety of applications in the lab. Get answers to your typical questions regarding their usage, reliability, and ergonomics, and discover new options with connected electronic devices and the Eppendorf Pipette Manager.

Watch the full presentation here: https://events.bitesizebio.com/precision-and-efficiency-at-your/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

But generating high-quality NGS libraries is a labor-intensive process that requires experience, precision, and accuracy. Optimal, thorough sample preparation upstream of the sequencing process is essential to ensure the best possible results.

In this episode of Listen In, get expert practical advice from Eppendorf on getting started with NGS in the lab, and discover how you can bypass pipetting-intensive protocols and automate your sample preparation with Eppendorf epMotion®—saving you time and effort and help you achieve reliable and reproducible sequencing results.

Watch the full presentation here: https://events.bitesizebio.com/introduction-to-liquid-handling-for-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

That's why it's such a pain when they break down.

But just because we use centrifuges every day doesn't mean there aren't important technical aspects to consider or no parameters you can tailor to your specific application.

In this episode of Listen In, learn everything you need to know before next using a centrifuge.

Let the experts from Eppendorf guide you through the difference between RPM and RCF, different rotor types, and dealing with aerosols. Plus, explore the best hardware for your intended experiment, get practical tips on prolonging their lifetime, and crucial safety information.

Watch the full presentation here: https://events.bitesizebio.com/essentials-in-centrifugation-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

However, alongside these unbelievably good papers, there are papers that are unbelievable due to the pervasive presence of statistical mistakes that undermine their credibility. Whether due to negligence, lack of expertise, or a rush to publish, these errors cast doubt on the validity of the reported results and conclusions.

In this episode of Listen In, JJ Orban de Xivry delves into the ten most common statistical mistakes that plague scientific research (Makin and Orban de Xivry, eLife, 2019).

With a focus on identifying these errors, he sheds light on the detrimental impact they have on the reliability of scientific findings. By identifying and rectifying statistical mistakes, researchers can ensure the foundation of their field of study.

Watch the full presentation here: https://events.bitesizebio.com/ten-common-statistical-mistakes-to

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, learn the key considerations when buying a new ULT freezer for your lab and the best tips and tricks to prolong its lifetime. Plus, get advice on reducing the energy footprint of your ULT freezer and keeping your samples safe inside them.

Watch the full presentation here: https://events.bitesizebio.com/how-to-keep-your-ult-freezer/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

While you probably realize that your liquid type and pipetting technique can influence your analysis results, you might be less sure about what pipettes to use when your assays involve challenging liquids and ultra-repetitive steps.

In this episode of Listen In, the experts at Eppendorf show you how to optimize liquid handling using simple tricks to ensure that "the pipette" does not influence your analysis results.

Discover the best approach to adopt for each liquid type, and learn how to implement superior pipetting techniques to improve the accuracy of your results.

Watch the full presentation here: https://events.bitesizebio.com/reproducibility-mastering-the-art-1/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, get a demonstration of the latest technology to image inside the cells of live animals.

Watch real-time multicolor sub-micron resolution image acquisition for dynamic cellular events, such as gene expression, cell trafficking, and cell–cell, and cell–microenvironment interactions in a range of organs.

And explore the latest research that uses real-time intravital imaging to investigate dynamic cellular-level pathophysiology of various human diseases to develop novel therapeutics.

Watch the full presentation here: https://events.bitesizebio.com/real-time-intravital-microscopy-ivm

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Any fraud in science is too much, but the reasons for it are complex.

In this episode of Listen In, join forensics detective Elisabeth Bik and explore the answers to these questions. See the work she has done uncovering scientific misconduct, and find out if you have a forensic eye as she presents genuine and manipulated data.

You will also hear examples of how scientific misconduct isn't always a deliberate attempt to mislead researchers and journal editors and learn the correct procedure for reporting a paper that you believe is suspicious.

Watch the full presentation here: https://events.bitesizebio.com/errors-and-misconduct-in-biomedical/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

You've done all the work and all the waiting—for nothing.

If this scenario sounds familiar, this episode of Listen In is for you.

Jessica Rowley, Flow Cytometry Facility Manager at Imperial College London, explains how to prepare flow samples optimized for your experiments, to save you time and avoid disappointment.

She has worked with hundreds of samples, so she is the perfect person to tell you how to optimize your flow cytometry protocol, from creating viable single-cell suspensions to staining, fixation, and improving data quality.

Plus, if you're new to flow cytometry, designing a new experiment, or want to improve your data quality, she has some advice on those, too.

Watch the full presentation here: https://events.bitesizebio.com/garbage-in-garbage-out-sample-prep
Browse all episodes of our educational webinar series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, our Content Creation Manager, Laura Grassie, unravels the complexities of consistent scientific writing and helps you steer clear of common pitfalls that make your work look sloppy. She introduces you to powerful, simple strategies that can significantly enhance the coherence, clarity, and, most importantly, the consistency of your research communication.

This episode is particularly beneficial if you're preparing for a crucial grant submission, planning your next research paper, or setting out to work on your thesis. The insights Laura shares will arm you with effective tools and techniques to achieve error-free, consistent scientific writing.

Don't let unorganized writing overshadow your extraordinary scientific work. Dive into this enlightening episode to discover the secret to consistent scientific writing that impresses everyone, from viva examiners to grant and paper reviewers.

Watch the full presentation here: https://events.bitesizebio.com/consistent-error-free-writing-tips/join
Browse all episodes of our educational webinar series here: https://listen-in.bitesizebio.com/

We've brought together a panel of experts and bioscientists with a wide range of experience to share their insights and help you carve out the best career possible.

Get advice and tools to forge a path that suits you—inside or outside academia.

Our panel shares their personal stories and insights into the various career paths available to bioscientists. Plus, gain valuable insights and a new perspective on the rewarding possibilities for your future.

With:
• Professor Stuart Maudsley, Odysseus Professor of Receptor Pharmacology, University of Antwerp
• Dr. Jane Luff, Director of Delivery, Our Future Health UK
• Dr. Axel Thomson, Business Development Executive, Edinburgh Innovations

Watch the full presentation here: https://events.bitesizebio.com/lab-meetings-live-career-paths-for/room

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Discover how to use freeze fracture and cryo-SEM workflow to answer research questions and learn its exciting applications in cell biology.

Watch the full presentation here: https://microscopyfocus.com/breaking-the-ice-freeze-fracture/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of the Listen In series, Dr. Johanna Ahlskog discusses the essential skills all reviewers and researchers need. She emphasizes the critical importance of the review process and offers general considerations when reviewing articles. Dr. Ahlskog also provides information on how to properly assess presented data and discusses issues surrounding image manipulation, including permissible versus problematic manipulation.

If you're interested in enhancing your manuscript reviewing skills, this episode is a must-listen. You'll gain valuable insights into the review process and learn how to navigate complex issues that may arise during the evaluation of a manuscript.

Watch the full presentation here: https://www.youtube.com/watch?v=8Nc5361cuTM
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, discover how the assets that make you a great scientist could also make you a great marketer in today's data-driven markets.

Learn the role of a marketer, how scientific skills translate into this career in today's data-driven markets, and how to adapt your talents to achieve success!

Join us, and explore the world of marketing and the dynamic and dominant role it plays in society.

To view the full presentation of this webinar, click here: https://www.youtube.com/watch?v=an0v67ZzxbQ

Browse all episodes of the Listen In Series here: https://bitesizebio.com/listen-in/

Dr. Seigel shares how to read and interpret a summary statement, ways to address critiques, and when you shouldn't resubmit. You'll hear real-world examples from previous grants to give you a flavor of what you can expect.

Watch the full presentation here: https://www.youtube.com/watch?v=kydPwDXehHA

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Dr. Seigel shares essential elements of writing the research plan section of your proposal, such as the essential items to include in your research plan, how to write a compelling narrative, and presentation tips to impress the reviewers.

Watch the full presentation here: https://www.youtube.com/watch?v=msUJGXAssDE

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Getting started is the first and potentially hardest step to writing a proposal that gets you funded.

Join Dr. Gail Seigel, a highly experienced faculty member with over 25 years of grant-writing and grant-reviewing expertise, as she shares her wisdom on kickstarting the proposal writing process.

Dr. Seigel guides you through the crucial first steps in writing a successful proposal that will impress funders. In this informative webinar, you'll learn how to target your proposal, bring the right people on board, and prepare the non-research sections of your proposal.

Watch the full presentation here: https://www.youtube.com/watch?v=MulKDhlV3yM

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Yet despite its frequent neglect, reproducibility is fundamental. It enables scientists to continue and expand upon existing work and builds trust in science and the scientific method.

So in what ways does the so-called reproducibility crisis hinder good research? What techniques and mindsets can we adopt to address it? How is it perceived by researchers in different areas of life science? And is poor reproducibility inevitable under current circumstances?

Join Max Green, founder of Panorama Laboratories and expert in research reproducibility, for deep dive into these questions and learn what insights Panorama Labs’ reproducibility survey gives us.

Whether you're a bench scientist generating data, involved in evaluating the success and efficacy of results, or responsible for allocating research resources, there is something for you.

To view the full presentation of this webinar, click here: https://www.youtube.com/watch?v=rNgFKwWP7xs

Browse all episodes of the Listen In Series here: https://bitesizebio.com/listen-in/

Let's face it; research papers are a pain to read. Their dry language and sheer density make crucial details hard to spot and obscure salient findings.

And if we're being honest, not every researcher is equally scrupulous. 

Sifting through mountains of papers to find the hidden gem that fills in that gap in our understanding is something we all have to do. But it doesn't have to be a burden. Join Dr. Amanda Welch and develop a systematic method to critically analyze scientific papers and discover an efficient way to fully understand their results and limitations.

Save time by knowing what to examine first, stay organized with a checklist that quickly varifies a paper's rigor, and learn the steps to take if you need more information.

Implementing these tools will enhance your critical thinking skills and build a strong foundation for your work.

To view the full presentation of this webinar, click here: https://www.youtube.com/watch?v=SwNydUyb0Ns

Browse all episodes of the Listen In Series here: https://bitesizebio.com/listen-in/

Scientists are under a lot of pressure. And many of us have had our working lives changed by recent global events in ways that have separated us from our colleagues and normal working routines.

Many of us feel strained and under pressure to maintain our productivity despite these changes. But how?

The counterintuitive answer is to slow down.

Join the founder of Bitesize Bio, Dr. Nick Oswald, and the commercial director, Kenneth Vogt, for a discussion on the benefits of going slow, and the practical ways to make it work.

To view the full presentation of this webinar, click here: https://www.youtube.com/watch?v=cSFC3Wx9DXw

Browse all episodes of the Listen In Series here: https://bitesizebio.com/listen-in/

Poster sessions are an amazing opportunity to get feedback on your research, make vital connections with others in your field, and hone your presentation skills.

But before you can showcase your amazing research findings, you need to design a poster that captures the attention of conference attendees. In this tutorial, we'll be sharing our top tips and handy hints for creating the best research poster possible.

We cover everything from choosing a compelling title to selecting the right fonts and colors to avoid, and where to stand when presenting your poster. You'll also learn about the importance of layout and positioning and how to make the most of your time when presenting your poster.

Whether you're a seasoned conference attendee or attending your first-ever event, this tutorial is sure to provide you with the knowledge and skills you need to create an eye-catching and informative research poster. So, don't miss out on this opportunity to improve your poster designing skills!

To view the full presentation of this webinar, click here: https://www.youtube.com/watch?v=wKHFzmQ52Js

Browse all episodes of the Listen In Series here: https://bitesizebio.com/listen-in/

There are many reasons you might want to start writing content online. While you may have experience writing papers, grants, and other content, writing specifically for the web requires different considerations.

In this episode of Listen In, you'll discover how writing for the web differs from traditional writing, get top tips for structuring and writing content for the web, and learn the basics of search engine optimization (SEO).

Watch the full presentation here: https://www.youtube.com/watch?v=_Iof0jXnwKY

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Join the Bitesize Bio editorial team for a fun and friendly introduction to grammar for scientists, as we cover everything from punctuation to the passive voice. We'll talk you through some of the most common grammar pitfalls in science writing and show you how to avoid them.

View the full presentation of this webinar here: https://www.youtube.com/watch?v=q8iA4mwMQn8

Browse all episodes of the Listen In Series here: https://bitesizebio.com/listen-in/

Well, look no further! In this exciting episode of Listen In, we take you on a journey exploring the many different avenues of science writing—from technical medical writing to fun and relaxed blog and journalism styles.

We'll also give you the inside scoop on how to break into the field, where to find job opportunities, and how to create a top-notch portfolio that will get you noticed. Whether you're itching to leave the lab or just curious about your options, this episode of Listen In has something for you!

Watch the full presentation here: https://www.youtube.com/watch?v=vivK55l0wH0

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Listen in to hear from Bitesize Bio's founder, Dr. Nick Oswald, and discover five simple but powerful principles you can use to carve out the career that best suits, excites, and rewards you inside or outside of the lab.

You'll discover:
• How to decide if the academic career path is for you.
• The key mindsets required to keep moving your career toward your desires.
• How Nick evolved his career from academic to industry research, then publishing, and finally to owning and running the business of his choice. And more importantly, what you can learn from that.

Watch the full presentation here: https://events.bitesizebio.com/5-principles-for-creating-a/

This webinar is presented by Bill Skarnes, Professor of Cellular Engineering at The Jackson Laboratory.

Follow Bill and The Jackson Laboratory on Twitter.

Click here to view the full presentation of this webinar.

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

This webinar is presented by Alexis Komor, Assistant Professor at The University of California, San Diego.

Follow Alexis and The University of California, San Diego on Twitter.

Click here to view the full presentation of this webinar.

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

This webinar is presented by Pia Johansson, Research Engineer at the Cell and Gene Therapy Core, Lund Stem Cell Centre, Lund University.

View the full presentation of this webinar here.

Follow Pia and Lund Stem Cell Centre on Twitter.

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

View the full presentation of this webinar here.

Follow Antony on Twitter.

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/.

Resources:
1 - https://www.linkedin.com/in/antony-adamson-52a0211a/
2 - https://www.bmh.manchester.ac.uk/research/platforms/genome-editing/

This webinar is presented by Anne Lodge, Chief Scientific Officer of Astarte Biologics.

Förster Resonance Energy Transfer (FRET) is an example of molecular environmental changes. The donor lifetime is shortened by the presence of the acceptor. FRET experiments thus benefit from FLIM information. The FRET-FLIM readout is independent of the donor or acceptor concentration making it the quantification assay of choice. Recently, FRET has been exploited to engineer sensors (FRET biosensors). FRET signal changes in these biosensors correspond to either binding or release of a ligand. How this type of readout can help uncover biological mechanisms was recently shown in a Nature Immunology article (Anzilotti 2019). The approach used in this article, and described in this webinar, opens the field to imaging in a range of situations in primary cells, where sensitivity and the need to avoid damaging the cells are both paramount.

For more information visit: https://bitesizebio.com/webinar/basics-of-fluorescence-lifetime-imaging-and-flim-applications-to-fret-biosensors/

– Capture weaker signals—and still get sound, reproducible data.
– Reach faster volumetric imaging without sacrificing resolution
– Increase data throughput for your imaging needs

Learn about the new Multiplex mode for parallel pixel acquisition with the ZEISS LSM 9 family and Airyscan 2. You can now acquire up to 8 superresolution image lines with high signal-to-noise rate in a single sweep. Capture dynamic processes, cellular signaling, molecular trafficking, and diffusion events with real-time superresolution and high SNR.

The new Multiplex mode for Airyscan 2 uses smart illumination and detection schemes for parallel pixel acquisition on a confocal microscope. Scientists can now capture weaker signals, keep their context, and get statistically sound data.

Extending Airyscan imaging to larger model systems with lower expression levels, the new Multiplex mode increases acquisition speeds even further. You get superresolution and a 4 times higher SNR compared to traditional confocals. This novel concept allows rapid volumetric imaging with unprecedented resolution beyond what is available in traditional confocal systems today.

Airyscan 2 provides new data handling concepts, providing 6.6 times smaller data sizes and 5 times faster image reconstruction times. Further, optimized real time acquisition strategies employed with the LSM 9 family enable faster scan speeds for Airyscan 2, allowing higher data throughput.

For more information visit: https://bitesizebio.com/webinar/zeiss-lsm-9-multiplex-factor-confocal-imaging/

– The complete workflow for in situ cryo-electron tomography
– How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae)
– How mapping these structures back into the native cellular environment reveals new molecular interactions that are only accessible by this technique (e.g., the binding of cargo to COPI-coated Golgi membranes and the tethering of proteasomes to the nuclear pore).

Cryo-electron tomography can visualize macromolecular structures in situ, inside the cell. Vitreous frozen cells are first thinned with a focused ion beam and then imaged in three dimensions using a transmission electron microscope. This transformative method has the power to revolutionize our understanding of cell biology, revealing native cellular architecture with molecular clarity.

for more information visit: https://bitesizebio.com/webinar/cryo-electron-tomography-for-cell-biology/

– How structural biology can change the face of your research
– The biological challenges of cancer research
– Advantages of cryo-EM for your research

Covering both biology and methodology, this webinar will explain how single-particle cryo-electron microscopy enables us to gain insight into cancer development through the detailed analysis of molecular structure.

In single-particle cryo-EM, hundreds of thousands of images formed by electron scattering of individual molecules or complexes are analyzed to derive their three-dimensional structure. Technological and computational advances have dramatically transformed the field of cryo-EM in the past years, enabling structural insights at near-atomic resolution into assemblies that had not been tractable using any other structural biology technique. Consequently, cryo-EM has become a mainstream method structural biology, with a multitude of new facilities and research groups being established all over the world within just a couple of years.

This webinar will address the roles that structural biology has been playing in cancer research, uncovering cellular processes involved in cancer development and protection, and guiding drug discovery efforts. The biological challenges of cancer research will be discussed, as well as the unique strengths of cryo-EM as an experimental approach towards these questions, briefly covering the methodology and procedures in sample preparation and data processing. We will illustrate these aspects with some of the latest research from the laboratory of Eva Nogales at UC Berkeley and assess the promises and challenges of cryo-EM in our fight against cancer.

For more information visit: https://bitesizebio.com/webinar/cryo-electron-microscopy-and-the-complexity-of-cancer/

In this webinar, we will explain how Elyra 7 with Lattice SIM takes you beyond the diffraction limit of conventional microscopy to image your samples with superresolution. Learn how to examine the fastest processes in living samples – in large fields of view, in 3D, over long time periods, and with multiple colors. The new Lattice SIM technology of Elyra 7 brings structured illumination microscopy (SIM) to a new level. Groundbreaking light efficiency gives you gentle superresolution imaging with incredibly high speed – at 255 fps you will get your data faster than ever before.

See how Elyra 7 lets you combine Lattice SIM with single molecule localization microscopy (SMLM) for techniques such as PALM, dSTORM and PAINT. Choose freely among your labels when imaging with resolutions down to 20 nm laterally. High power laser lines allow you to image your sample with ease, from green to far red.

Elyra 7 is also very flexible: you can employ a wealth of contrasting techniques and combine them with optical sectioning. The new Apotome mode gives you superfast optical sectioning of your 3D samples. All that, plus Elyra 7 works seamlessly with your ZEISS SEMs in a correlative workflow.

For more information visit: https://bitesizebio.com/webinar/revealing-the-fastest-processes-in-superresolution-with-lattice-sim-and-zeiss-elyra-7/

In this webinar you will get tips and tricks to optimize the workflow of your array tomography:

– Fast and precise trimming of the sample block-face
– Adhesion of a single section to create ribbons
– Automated serial sectioning with the ARTOS 3D ultramicrotome
– Acquisition and processing of a 3D SEM dataset
– Segmentation and 3D reconstruction of cells
– Interpretation of 3D reconstructions

For more information visit: https://bitesizebio.com/webinar/array-tomography-for-sem-3d-reconstruction/

– Improved vitrification: Specimen vitrification without synchronisation fluid
– Vitrification strategies: Optimized freezing for different techniques
– Light and electrical stimulation: Dissect cellular processes with millisecond precision
– A look into the future: freezing of crystals

Plunge freezing and cryo imaging of proteins and complexes have revealed new details in understanding the machinery of the cell and how molecules are involved in cellular processes. However, most eukaryotic cells and tissue samples cannot be plunge frozen because of the rapid decay of the cooling rate within the sample during freezing. High pressure freezing, on the other hand, is currently the main approach to vitrify larger samples (up to 200 µm) and to capture the intrinsic changes in fine structure or cellular dynamics. To further improve its cryo solutions, Leica developed a new cryo platform: the EM ICE. This new generation cryo platform combines speed, reliability and flexibility to facilitate research in various scientific fields.

The EM ICE allows users to freeze samples within milliseconds and even permits the combination of high pressure freezing with optogenetics and electrophysiology.

For more information visit: https://bitesizebio.com/webinar/expanding-the-limits-of-electron-microscopy-sample-preparation-with-the-leica-em-ice-high-pressure-freezer/

In this webinar, you will learn:

1. How CRISPR/Cas9 can be used to “flag” alterations and mutations in DNA, rather than cut it
2. How pairing atomic force microscopy (AFM) with optical equipment found in DVD players can be used to map DNA at a faster rate than traditional DNA sequencing
3. Applications of this technology, particularly in relation to discovering and diagnosing genetic diseases

Since the diameter of the Cas9 molecule is greater than that of DNA, they are easy to locate along the DNA strand. Taking advantage of this, Jason’s lab reported approximately 90% Cas9 binding accuracy to DNA molecules under optimized conditions. The alignment of single-molecule maps with nanoscale resolution becomes far more computationally straightforward than if labels are localized with multi-kb ambiguity. This process yields reduced processing time and cost for assembling a consensus map. Given its single-molecule sensitivity, approximately 15 bp accuracy, and no amplification requirement, Dr. Reed’s novel method is amenable to small sample sizes. This proves to be an advantage in clinical situations where obtaining the almost 10 ?g of DNA required for single-molecule sequencing is extremely difficult—if not impossible!

For more information visit: https://www.youtube.com/watch?v=58LKOQSmbTY

In this webinar, you will learn:

– The advantages of using HDR versus recombinant viral vectors when modifying T cell genomes
– How long double-stranded and single-stranded DNA can serve as a non-viral HDR template
– A novel method that allows for the insertion of large DNA sequences (>1Kb) without a virus!

Current efforts at reprogramming T cells for therapeutic purposes rely on using recombinant viral vectors. Unfortunately, viral vectors do not target transgenes to specific genomic sites. Moreover, the manufacturing and testing of effective viral vectors is often a lengthy and expensive process, which slows research progress and clinical use. However, recent studies have shown that re-engineering T cells in a specific and efficient manner is possible using homology-directed repair (HDR).

For more information visit: https://www.youtube.com/watch?v=CCXY9GNn-2w

– How to choose the best method to extract your nucleic acids

– Which method you should chose to determine nucleic acid quality

– How to avoid common pitfalls for both extraction and quality control

Virtually all experiments in the molecular biology lab require high quality, pure nucleic acids as a starting material. This sounds simple enough. However, as always, the trouble is in the details. There are several methods to isolate your nucleic acids. But which one is best for your experiment? How do you determine nucleic acid quality? And why does it seem to not be working?

Watch this webinar to find out the answers. Victoria will breakdown the nucleic acid purification process and how to match the right method to the right type of nucleic acid. She will also show you how to avoid common pitfalls and contaminants in isolation and quality control. This webinar is essential for anyone in a molecular biology lab who wants better results in downstream experiments!

Fore more information visit: https://bitesizebio.com/webinar/nucleic-acids-101-confirming-their-quality/

– How to best prepare your specimen for live cell isolation by laser microdissection
– How to optimize your laser microdissection workflow
– How to avoid common pitfalls of this technique

Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections, laser microdissection may be applied for live cell isolation. Unlike other well-established and widely used techniques for live cell isolation and single cell cloning—such as FACS, MACS, cloning by limited dilution, and so on—laser microdissection allows for capturing live cells and cell colonies without their detachment from the carrier. In other words, there is no need to prepare a single cell suspension before the isolation procedure using mechanical and enzymatic dissociation, which can affect cell fate after plating. This feature of laser microdissection is desirable for stem cell research. We established a simple strategy for the efficient live cell isolation using the Leica Laser Microdissection platform. We were able to demonstrate not only colony formation from the isolated samples containing live cells, but also single cell cloning. In this webinar, specimen preparation, laser adjustment, overall workflow, and limitations on live cell isolation by laser microdissection are discussed.

For more information visit: https://bitesizebio.com/webinar/live-cell-isolation-by-laser-microdissection/

– How to overcome issues with stage drift and crosstalk between channels
– How to obtain reliable images and reproducible quantification results
– How to proceed with advanced image analysis after restoring images

During fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of experimental trouble. Additional aberrations, such as stage drift, and crosstalk and chromatic aberration between channels, can also affect the imaging. Huygens image deconvolution and restoration is a proven method to revert these issues and recover a more realistic representation of the original object. After restoration of the image, you can proceed with the advanced Huygens analysis options for colocalization, object measurements, and tracking.

This webinar will illustrate how you can make optimal use of the complete Huygens workflow to obtain reliable images and reproducible quantification results, focusing on the advanced analysis options offered by the Huygens software.

For more information visit: https://bitesizebio.com/webinar/reliable-quantification-of-fluorescence-images-from-raw-data-to-reproducible-results/

– When and why you should use the different types of ELISAs– direct, indirect, sandwich, competitive/inhibition ELISA
– Major considerations for developing your ELISA
– Tried and tested tips for you to perform a successful ELISA

The ELISA is one of (if not the most) common techniques in biology and biochemistry laboratories. You can use an ELISA to detect minute amounts of protein for medical diagnostics, for testing food for common allergens, and in toxicology screens screen for certain drugs. While the overall premise of an ELISA is simple, as with any assay, its success hinges on the experimental set up.

In this webinar, Omonse will give you a crash course on that experimental set up—from start to finish. She will discuss the different types of ELISAs, the major considerations in development of the assay, and troubleshooting—including tips and tricks. With this webinar, you will be able to ensure that your ELISA provides reproducible and publishable results!

For more information visit: https://bitesizebio.com/webinar/crash-course-developing-a-fool-proof-elisa/

In this webinar, you will learn:

– How to use genome-wide CRISPR screening for mutant discovery
– How to create a highly diverse, sgRNA library from Twist Bioscience for targeted, subtle mutations
– How knowledge of the structure-function relationships of PARP1 mutants can inform treatment of cancer patients with these drugs

For more information visit: https://www.youtube.com/watch?v=opfEytUeJLI

Proliferation:

3H-Thymidine Uptake
Bromodeoxyuridine Uptake (BrdU)
ATP Luminescence
Fluorescent Dye Reduction (CFSE)
Cytokine Measurement:

Multiplex vs. Single Cytokine
Choice of Cytokine (IFNg, TNFa, IL-6, IL-1?, etc.)
Kinetics of Cytokine Release
Surface Antigen Expression:

CD69, CD25, PD-1, etc.
Combine with CFSE, Ki67 or BrdU
Kinetics are Important
Cytotoxicity:

Two-Label Flow Cytometry
Calcein AM Dye Release
Luciferase Transduced Targets
Annexin V

Activation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer, and disease.

There are many ways to measure immune cell activation, and they all have utility. Methods can be grouped into four main categories: Proliferation Assays, Cytokine Measurement, Surface Antigen Expression, and Cytotoxicity.

For more information visit: https://www.youtube.com/watch?v=ocW-pNVS_gE

• Low (1-5 genes) vs medium (~300 genes) throughput experimental design
• Pros and cons of self-designed vs “off the shelf” assays
• How to set up your wet lab experiments start to finish
• Downloadable example step-by-step experiments with real data analysis and tutorial
• Biological considerations (time series data, cell population frequency changes + more)
• Examples of these techniques in publications
• Common pitfalls and how to avoid them
• Limitations of the technique
• MIQE and publication standards

Whether you are interested in a few genes or a few hundred, join Matthew Mule as he takes you through the necessary steps to validate expression levels of target genes using qRT-PCR with single gene assays and other medium-throughput platforms.

For more information visit: https://bitesizebio.com/webinar/target-gene-validation-quantitative-rt-pcr/

In this webinar, you will learn:

• How to choose between immortalized cells and primary cells for your assay
• How to avoid sources of bias in your cell-based assays
• How to use high throughput assays, so you can achieve greater reproducibility

By now, you have heard about the reproducibility crisis—the inability of scientists to reproduce experimental results. This crisis spurred journals to institute new requirements for publication and funding agencies to introduce more stringent rigor and reproducibility criteria. In this webinar, Vicki will address an often overlooked source of experimental variability: state of your cell lines and their external conditions.

The good news is that many of these factors can be addressed through assay design. She will show you how to move from poor reproducibility of so-called “artisan experiments” to the use of standard conditions for your cell lines and use of automatic systems for high throughput screens.

In this tutorial, you will find:

• How you can run one scRNA-seq experiment with numerous protein markers in parallel
• How you can increase your recovery of single cells (up to four times!) per experiment
• How you can link phenotypes to transcriptomic profiles—with higher throughput methods!

The last few years have seen the scale of single cell RNA-seq experiments increase exponentially, greatly enhancing our understanding of cell biology in development and disease. It is now feasible for researchers to characterize thousands of single cells in one experiment. However, important hallmarks of immune cell states are often not detected in scRNA-seq experiments. While lower throughput methods previously allowed researchers to link phenotypes or protein expression to transcriptomic profiles, the increase in scale of modern droplet-based methods resulted in a loss of such addressability.

Here we describe two recently developed applications that utilize antibody-conjugated oligonucleotides to enhance existing scRNA-seq platforms.

1. CITE-seq, which allows measurement of a potentially unlimited number of protein markers in parallel to transcriptomes.

2. Cell Hashing, which enables sample multiplexing, robust multiplet detection and super-loading of scRNA-seq platforms, allowing confident recovery of 4 times as many single cells per experiment.

Reagents for performing these assays, under the name TotalSeq™ are now available from BioLegend.

For more information visit: https://www.youtube.com/watch?v=A2mcnnufkOs

In this webinar, you will learn:
1. The epigenetic differences between microbial and human/animal genomes
2. The use of restriction endonucleases to enrich for either pathogen genomes or the human genome from complex populations.
3. How the use of enrichment and concentration can improve qPCR sensitivity
4. The use of qPCR to validate enrichment from complex samples
5. The use of NGS to validate pathogen enrichment from complex samples

Join Dr. Allyn Forsyth as he describes how successful NGS analysis of complex microbiome samples can lead to the development of non-invasive colorectal cancer screening, as well as monitoring of disease states within other cancers and Alzheimer’s Disease.

For more information visit: https://www.youtube.com/watch?v=KwevU0yiSx8

For more information visit: https://bitesizebio.com/webinar/super-resolved-sted-spectroscopy-new-insights-into-molecular-membrane-dynamics/

– the sources of bias throughout the microbiome analysis workflow
– practical solutions for troubleshooting your techniques
– new technologies to achieve the most representative and unbiased microbiome profiles

Join Dr. Sven Reister as he guides you through a typical workflow for analysis of microbial community profiles of complex and low biomass microbiomes, and learn how to get the most complete, unbiased microbiome profiles from even your most challenging samples.

For more information visit: https://www.youtube.com/watch?v=s9Y9gO7RsE4

We present the Leica SP8 FALCON, a new high-end confocal instrument with built-in very fast FLIM capabilities. The instrument applies a novel technology to records photon arrival times at up to 80 MHz (160 MHz if two detectors are used) using the built-in spectral HyD detectors. To arrive at such high counts rates, the instrument uses real-time 10 GHz sampling and analysis algorithms that render it largely immune to pulse pile-up. Falcon FLIM has been fully integrated in the LAS-X software, allowing convenient acquisition of FLIM time-lapses, stitched FLIM overview images, FLIM XYZ-stacks and spectral FLIM stacks, among others, as well as combinations thereof.

We scrutinized the performance of the SP8 FALCON and applied it to record agonist-induced changes in concentration of second messengers such as cAMP and Ca2+ with sub-micrometer precision at high speed- on the order of several (512×512) frames per second (FPS). To detect cAMP by FLIM, we used our newest generation of EPAC-based dedicated FLIM sensors, which use mTurquoise2 as a donor and a tandem of two monomeric dark Venus proteins as acceptors. Reducing the image format to 128×128 pixels, we acquired good quality FLIM time-lapses at 25 FPS (83 FPS using resonant scanning), allowing detection of even very fast signaling events, including e.g. the activation of G-proteins and Ca2+ sparks. The large numbers of detected photons reduce pixel-to-pixel variability in the calculated lifetimes. Finally we demonstrate that the SP8-FALCON is ideal for fast FLIM screening applications in both fixed- and live-cell formats.

For more information visit: https://bitesizebio.com/webinar/precise-and-lightning-fast-spectral-fluorescence-lifetime-imaging-at-video-rate-integrated-in-a-high-end-confocal-microscope/

You will learn a fast, efficient and flexible approach to 4D microscopy image analysis, which yields high quality images and results.

We’ll cover:

• How to handle and process large image data quickly

• How to detect cells, nuclei, membranes and cellular structures easily with interactive image analysis including the use of virtual reality for editing

• How your research can benefit from using server environments for higher throughput and collaboration with other researchers.

Join Dr. Chris Zugates as he takes you through a typical workflow of image analysis, learn how to easily process your data and find the objects of interest quickly and interactively creating meaningful results for publication.

Chris will then show how arivis can support you through the whole workflow from efficient image acquisition up to the presentation of your work via Web or via Virtual Reality to the scientific community.

For more information visit: https://www.youtube.com/watch?v=HxpBpFyOxDo

In this webinar on DNA assembly, you will learn:

• Key concepts in DNA assembly
• The importance of analyzing combinatorial libraries of genetic designs for natural product biosynthesis
• Emerging areas of research

Join Dr. Michael Smanski as he discusses his group’s work in engineering natural products, specialized metabolites that are not necessary for growth or reproduction of the organism(s) that produce them. By focusing on how combinatorial DNA assembly strategies can be used to interrogate and optimize refactored gene clusters, Dr. Smanski aims to frame an example application of DNA assembly for the production of a natural product with promising pre-clinical bioactivity.

– How to improve the quality of your images with the new Batch Express
– How to increase resolution and signal of all your fluorescent microscopy data
– How to deconvolve your new acquired images instantly
– If you are interested in improving your fluorescent microscopy images, without manual intervention, then this is the webinar for you!

The Huygens software is considered the gold standard for deconvolution and restoration of microscopy data. Its high-quality deconvolution is now accessible with a fully automated image-processing pipeline within the new Batch Express option. You simply have to select the desired Image Feeder folder and the images in the folder will be automatically deconvolved by the Batch Express within seconds. The Batch Express supports a wide range of microscope types (widefield, confocal, spinning disk, multiphoton, STED, and a variety of SPIM/Light Sheet systems) and file formats. Compatibility with your image files is not an issue. After this webinar, your images will look better than ever before!

The difference and benefits of LASSO cloning over NGS
How LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbiome genomes
How this technology can impact our understanding of disease, drug discovery, precision medicine, and so much more
Join Dr. Lorenzo Tosi as he describes LASSO cloning, a technique that will allow for the expression of an entire set of proteins from any organism in more rapid and cost-effective manner.

The development of tools and techniques designed to sequence and understand genomes has advanced our understanding of the world at large. However, our ability to analyze the function of expressed molecules from these genomes is lagging. Even with the advent of next generation sequencing (NGS), which has changed the landscape of genomic sequencing through increased accuracy and speed, our experiments are still limited by the length of DNA targets being read.

For more information visit: https://bitesizebio.com/webinar/long-adapter-single-stranded-oligonucleotide-lasso-probes-for-massively-multiplexed-cloning-of-kilobase-sized-genome-regions/

For more information visit: https://bitesizebio.com/webinar/new-2d-superresolution-mode-zeiss-airyscan-fast-gentle-confocal-imaging-120-nm-resolution/

– How flanking homology DNA assembly methods work
– How to use web-based software to design experimental methods for flanking homology DNA assembly methods
– How synthetic DNA fragments fit in to the DNA assembly process

Dr. Nathan Hillson will discuss methods in flanking homology DNA assembly, including Gibson, In-Fusion, and yeast TAR assembly—amongst many other related methods. Current DNA assembly methods offer many advantages over traditional (multiple cloning site, digestion/ligation) approaches, including the ability to assemble multiple fragments at once, the lack of a necessary specific restriction enzyme, and time commitment.

One part of DNA assembly is designing these experimental methods. To that end, Dr. Hillson will provide a demonstration of how to use web-based software to automate and optimize the design of protocols for these methods.

Finally, Dr. Hillson will explain how synthetic DNA fragments fit in to the DNA assembly process and how this relates to your work.

For more information visit: https://bitesizebio.com/webinar/flanking-homology-dna-assembly-protocol-design-software-synthetic-dna/

Gain insight in to how to use x-ray microscopy to analyze biological samples
Learn the basics about sample preparation for x-ray microscopy
Understand the benefits and drawbacks to different imaging conditions
X-Ray Microscopy (XRM) is a relatively new technique that combines the geometric magnification of traditional micro-CT with the optical magnification of light microscopy. Using XRM you can image the internal structure of objects with fine resolution without destroying the sample. For example, the Zeiss Versa XRM system allows an unprecedented view inside samples varying in size from the mesoscale (cm) to the microscale (µm) at consistently sub-micron image resolutions.

This webinar will focus on biological applications of X-Ray microscopy. We will cover imaging calcified structures, such as bone, to soft tissues, like the intervertebral disc. You will also learn about visualizing blood vessels using vascular tracing agents. In addition, we will cover the basics of sample preparation along with the pros and cons of different imaging conditions. Finally, we will give you a sneak view into using XRM to spatially target, in three-dimensions, tissue specific structures in a whole organism for 3D ultrastructural imaging using Focused Ion Beam – Scanning Electron Microscopy (FIB-SEM) using the ATLAS 5 Correlative Workspace.

For more information visit: https://bitesizebio.com/webinar/biological-applications-x-ray-microscopy-correlative-xrm-fib-sem-imaging/

For more information visit: https://bitesizebio.com/webinar/large-volume-serial-blockface-electron-microscopy-imaging/

-The main aspects covered in the webinar include:
-How to design an effective optocardiography system and select optimal fluorescence probes for your research needs using open source hardware and software.
-Troubleshooting tips for reducing noise and motion artifacts during data acquisition
-Advanced optocardiography: multi-parametric, panoramic and transillumination imaging.

Optocardiography: Optical Imaging of Cardiac Physiology

Optical mapping is a technique that is widely used to study heart rhythm disorders, as it offers a higher spatial and temporal resolution on cardiac tissue over traditional electrical mapping. Join Dr. Igor Efimov as he takes you through the steps involved for designing a real-time physiological imaging system as well as a novel, easily-reproducible panoramic imaging system. He will explain the history of optical mapping, basic principles and techniques for obtaining high-quality signals, and useful troubleshooting advice for both the beginner and experienced researcher. The video will take a look at optocardiography of whole mammalian hearts, isolated regions of the heart, and organotypic human cardiac slices. Viewers will also discover RHYTHM, an open-source software for optical mapping data analysis that was developed by the Efimov lab. Finally, the webinar will display representative results of optocardiography studies carried out in different animal models and human heart.

For more information visit: https://bitesizebio.com/webinar/optocardiography-optical-imaging-cardiac-physiology/

We recently developed a technique that allows localized proteomics of microscopic regions of interest, such as specific cell types or neuropathological features of disease. This technique uses laser capture microdissection (LCM) to isolate regions/cells of interest followed by label-free quantitative mass spectrometry (LC-MS). Importantly, we optimized this technique to use formalin-fixed paraffin embedded (FFPE) tissue, so that archived human tissue specimens collected at autopsy could be used. This is a particular advantage of our methodology, as the vast majority of human tissue specimens are FFPE blocks, which are currently an underutilized, but exceptionally valuable resource for medical research. We have successfully used this technique to analyze the proteome of neuropathological features that define Alzheimer’s disease (amyloid plaques and neurofibrillary tangles), as well as specific populations of neurons that are vulnerable in AD. Going forward, the use of localized proteomics has the potential to greatly increase our understanding of the molecular mechanisms that underlie AD. More broadly, this technique could be used to analyze regions or cells of interest isolated from any FFPE tissue, and therefore could be widely used to examine disease pathogenesis across a broad spectrum of diseases.

For more information visit: https://bitesizebio.com/webinar/conduct-localized-proteomics-microscopic-regions/

In this webinar you will learn:

– Available tools to accurately sort vitrified protein complexes into distinct populations
– How to use those classifications to assess the influence of specific perturbations upon conformational state at level of individual protein complexes.
– How to characterize how specific components that influence the equilibrium between conformational states

For more information visit: https://bitesizebio.com/webinar/harnessing-power-heterogeneity-cryo-electron-microscopy/

For more information visit: https://bitesizebio.com/webinar/em-cellular-connectomics-zeiss-multisem/

Introduction to synthetic biology – what it is and what it’s not
Popular applications for synthetic biology in research
The pros and cons of synthetic biology
Tips and resources for getting started in this field
The Sky Is the Limit with Synthetic Biology!
The notion of synthetic biology began to receive attention in the 1970s with the discovery of restriction endonucleases. However, the field had its real debut in 2000, when research groups in the US used synthetic biology techniques to devise biological circuit devices controlling gene expression and biological clocks by combining different genes in E. coli.

Although no standard definition exists, synthetic biology is generally understood to be a graft of engineering and biology, and this has led to some confusing and unhelpful metaphors. In this webinar, Dr. Richardson will introduce you to the interdisciplinary world of synthetic biology, busting some of the popular myths about this field—DNA is not a programming language, and cells are not compilers or circuit boards!

Dr. Richardson will also tell you about how synthetic biology differs to recombinant DNA technology, and you will get a solid overview of where and how synthetic biology can be used within research. You will receive tips and resources to help you embark on synthetic biology. The webinar will finish with open questions and future perspectives for this exciting and fast-growing field.

For more information visit: https://bitesizebio.com/webinar/engineering-cell-behavior-synthetic-biology/

They must be expressed at sufficiently high levels in the organism of choice
They must be purified to high purity from the expression system in an immunogenic form
They must induce potent immune responses
Dr. Patrick McAtee will take you through these vaccine development steps using the nucleoside hydrolase antigen from Leishmania donovani as an example. He will demonstrate how his lab cloned and expressed the full-length, 36-dKa protein. He will discuss purification of the protein to >99% purity using anion exchange and gel filtration chromatography. He will also talk about the steps taken to ensure protein integrity and enzymatic activity using lithium dodecyl sulfate polyacrylamide gel electrophoresis (LDS-PAGE), mass spectrometry (MS), and enzymatic assays.

Dr. McAtee will then take you through in vivo testing of the vaccine candidate including analyzing antibody levels from mice immunized with the protein alone or in a stable emulsion with glucopyranosyl lipid adjuvant (GLA-SE). He will describe characterization of the type of cellular immune response induced by the protein. Finally, he will demonstrate protective efficacy in mice challenged with Leishmania mexicana.

For more information visit: https://bitesizebio.com/webinar/development-of-a-potential-recombinant-protein-vaccine-in-e-coli/

The main points in the webinar will include:

How to design guide RNAs using online tools specific to the genome and application of interest.
Tips and practical advice to assist you in choosing and optimizing a CRISPR/Cas9 delivery system.
How you can create precise mutations using homology-directed repair, including template design and cleavage site
An Essential Guide to CRISPR/Cas9 Editing Efficiency
While CRISPR/Cas9 editing is utilized in a wide variety of cell types, editing efficiency continues to pose a challenge to researchers. Join Dr. Allison Mayle, as she shares best practices for increasing CRISPR/Cas9 editing efficiency. Viewers will discover online tools to aid in CRISPR/Cas9 design and delivery and tips for optimizing your CRISPR/Cas9 experiments.

In this webinar, Dr. Mayle will review the factors influencing genome editing, including target sequence selection and CRISPR delivery methods. A comparison of plasmid and viral vector delivery will be provided, as well as an introduction to DNA-free CRISPR/Cas9 ribonucleoprotein reagents. Additionally, Dr. Mayle will cover best practices for CRISPR knock-in mutagenesis via homology-directed repair (HDR) and applications available from new Cas9 protein variants.

For more information visit: https://bitesizebio.com/webinar/guide-crisprcas9-delivery-maximize-editing-efficiency/

Principle behind multiplex PCR technology
Popular applications of this technology
How to set up this reaction
Advantages and disadvantages
Multiplex PCR Can Benefit Your Research
Dr O’Hanlon Cohrt will discuss the history of multiplex PCR, how the technique works, and how to set up a reaction. She will also provide advice for optimization of primers and how to detect your targets.

Multiplex PCR technology simultaneously detects multiple nucleic acid targets in a single reaction. This method is a straightforward and efficient solution for bypassing challenges associated with limited template material. As an added bonus, this technology is cost effective because fewer reactions are needed to detect disparate targets.

Karen will show you the real beauty of multiplex PCR— how you can use very little template or sample in each experiment. She will also share the applications of this technology, which include forensic science, pathogen identification, and genetic testing—especially in cancer.

For more information visit: https://bitesizebio.com/webinar/multiplex-pcr-technology/

Define open access and Creative Commons licensing and dive into the numbers about open access (journals, articles, fees)
Discuss the pros and cons of open access and dispel some myths
Describe how funder and government mandates will affect the open access movement and individual researchers

For more information visit: https://bitesizebio.com/webinar/changing-landscape-open-access-publishing/

The main points we will cover are:

The reasons that could color reproduction go wrong in your microscopy images
How to correct your microscopy images with inappropriate color reproduction
How to be ethical with the color in your microscopy images
Advice on Color in Microscopy Images
Mark Clymer, an expert in microscopy imaging, will guide you through the complicated area of color reproduction in microscopy. He will offer advice and tips on how to get the most from your microscopy camera.

Color imaging is everywhere today and is often taken for granted. Images are instantly captured on cell phones, tablets, web cams, and microscopes. Pictures are shared with friends and colleagues even faster. We thrive on instant gratification. For some of us, our images are simply a snapshot in time—a selfie in front of a landmark, an amazing meal presentation, a pic showing viable cells in culture. But for others, images need to be masterpieces, expressing not just artistry but scientific discovery.

Regardless of your philosophy, we are at the mercy of the technology: cameras AND software. So as scientists, how do we ensure that we capture and communicate images that are worthy of our research? In this webinar, you’ll learn about how color in images goes awry, and what you can do about it. And there’s a twist at the end about an often overlooked villain in this whole scheme.

About the Presenter:
Mark Clymer is a veteran of the microscopy industry, establishing his fundamental skills in biotech and the drug discovery labs at Sanofi, and honing those skills as product manager at Olympus, managing the core microscope product line in the Americas. Along the way, Mark attended microscopy-based courses at the Marine Biological Laboratory in Woods Hole, MA, and now shares his expertise with students during the Immunohistochemistry & Microscopy course held each March at the MBL.

Mark offers consulting services to microscopy and biomedicine-based companies, and is the former Director of Marketing for the Datacolor Scientific division of Datacolor Inc., the Swiss-headquartered market leader in color management for industry and photography. He is the author of numerous articles and blogs on microscopy and color management. You may have seen some of his posts to microscopy and imaging-based groups on LinkedIn. Mark is also an independent microscopy and spectrophotometry sales specialist for Laxco Inc.

Mark invites you to look him up on LinkedIn

For more information visit: https://bitesizebio.com/webinar/color-image-quality-control-microscopy/

In this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components.

We will cover:

How quality custom reagents in small sizes will make your panel design easier
How you can receive these reagents fast and without traditional wait times for manufacturing
How you can be confident that quality control is maintained for these custom reagents
Incorporating New Dyes to Simplify Panel Design
Dr. Jorg Ruhrer, Director of Research Reagents at BD Bioscience will take through how to overcome a major limitation in multicolor panel design for flow cytometry experiments—limited antibody-fluorochrome combinations. Join him in this webinar as he shows you how custom reagents provide increased options in your multicolor panel design.

Flow cytometry continues to be a critical tool for the analysis, characterization, and isolation of single cells from a heterogeneous cell population. The rapid introduction of dyes based on Sirigen technology has dramatically increased the total number of parameters that can be analyzed simultaneously from a single sample. Using these dyes coupled with recent advancements in flow cytometers, it is now possible to probe more than 28 different fluorescence parameters simultaneously, allowing for a much deeper biological understanding of a sample.

However, in spite of these advancements, one of the major limitations to designing optimal multicolor panels is the unavailability of many reagents and specificities in these new fluorochromes. To address this need, BD Biosciences has launched a new product line that will give scientists immediate access to hundreds and eventually thousands of new antibody/bright dye combinations. These new combinations will give the flexibility that you need to design a multicolor panel without compromising on results.

Jorg will discuss how these quality reagents will enable easier panel design, resulting in robust high content panels that will generate quality data to drive ever deeper scientific discoveries.

For more information visit: https://bitesizebio.com/webinar/incorporating-new-dyes-simplify-panel-design/

The main points that we will cover are:

Practical advice and methods for sample collection and preparation for your genotyping experiments
An overview of the available data analysis methods for your genotyping results
Examples of pathway analysis methods for determining potential mechanisms within your system
Advice, tips, and applications for your genotyping experiments
Dr. Ania Wronski, a breast cancer researcher from Tufts University, will guide you through the process of investigating the genotype of tumor cells. Join her in this webinar as she takes you from sample collection to data analysis.

The American Cancer Society predicts that 1.7 million new cancer cases will be diagnosed and almost 600,000 cancer deaths will occur this year in the United States alone. These staggering numbers prompted the creation of the National Cancer Moonshot initiative in the US and contributed to the prominent position of cancer research in the European Union’s Horizon 2020 project.

Recent evidence suggests that cells within a particular tumor are not all genetically identical. Most tumors exhibit some degree of cellular heterogeneity. While most cells within the tumor possess one or more dominant mutations, sometimes there is a subpopulation with different ones. Researchers hypothesize that these subpopulations might be responsible for resistance to therapeutic agents. Discovering the genotype of tumor cells might yield new therapeutic targets to aid in the fight against multidrug resistance of cancer.

Ania will use her own research as an example and give you a broad strokes overview of the steps necessary to interrogate the genotype of tumors. She will lead you through the collection and preparation of samples and discuss available data analysis methods. Finally, she will share examples of pathway analysis that can be used to discover potential mechanisms.

For more information visit: https://bitesizebio.com/webinar/practical-advice-genotyping-cancer-cells/

For more information visit: https://bitesizebio.com/webinar/all-about-mirnas-practical-tips-advice-and-applications/

For more information visit: https://bitesizebio.com/webinar/tools-for-analyzing-genetic-variants-from-sequencing-data-case-study-short-tandem-repeats/

For more information visit: http://bitesizebio.com/webinar/26901/5-actionable-steps-for-transitioning-smoothly-to-a-non-faculty-job/

For all the widespread uses of NGS, there are a variety of ways to end up at your destination, along with some unique challenges along the way. To determine the path, it is best to begin with the end in mind.

For more information visit: http://bitesizebio.com/webinar/26429/next-generation-sequencing-overview-step-by-step-guide-to-ngs-workflow/

For more information visit: https://bitesizebio.com/webinar/how-bitesize-bio-gets-progressive-companies-to-help-you-the-working-scientist/

We have developed new tools based on fluorogenic forced intercalation (FIT) probes for RNA detection, quantification and interference in biological samples. The probes contain a thiazole orange (TO) dye introduced at a position normally occupied by a nucleobase. Upon binding to target nucleic acids, the TO dye increases its quantum yield and brightness substantially (greater than10 fold). These probes detect mRNA in a rapid, wash-free FISH setup using conventional wide-field microscopy. It is an ideal tool for RNA localization screens.

Nuclease resistant FIT probes containing a locked nucleic acid adjacent to the TO dye are bright and contrasted enough for use in live imaging. These probes can also be designed to target functional elements of RNAs to test the role of those in RNP biogenesis.

Absorption and emission spectra of TO are sufficiently different from EGFP to enable high sensitivity and specificity RNA-protein co-localization analysis, even with super-resolution, to study the RNA interactome. LNA modified FIT probes are excellent subjects for STED microscopy as duplex formation greatly increases their fluorescence lifetime.

For more information visit: http://bitesizebio.com/webinar/25961/deciphering-steps-of-mrnp-assembly-in-developing-oocytes-using-super-resolution-microscopy/

Cutting-edge single-cell analysis tools for exploring the genomic, transcriptomic and proteomic states of both tumor and related immune cells are enabling researchers to understand system heterogeneity, identify cells with previously unrecognized phenotypes and elucidate important therapeutic mechanisms.

Jonathan Irish will present his work on human solid tumor cytomics, which is revealing novel melanoma and immune cell subsets. He will highlight high-content single-cell approaches developed by his lab for systems immunology and cancer biology studies of human solid tumors, including melanoma and brain cancer. Dr. Irish will also discuss technical and biological quality controls, computational analysis and the strengths of combining single-cell approaches, such as mass cytometry, phospho-flow, imaging, transcript profiling and sequencing. These cytomic approaches are especially powerful for dissecting cellular mechanisms of treatment response, monitoring key biomarkers and precise targeting of clinically relevant cell subsets.

Manisha Ray will guide you through the newest Fluidigm technologies for single-cell analysis at the genomic, proteomic and transcriptomic levels.

For more information visit: http://bitesizebio.com/webinar/26112/know-the-players-combinatorial-single-cell-approaches-to-explore-the-complexity-of-biologic-systems/

After obtaining precious specimens, it is important to not only store them for easy retrieval, but is also vital to treat the samples so there are no complications for downstream uses. Tissues, blood, and cells all have unique requirements for maintaining specimen integrity.

For more information visit: http://bitesizebio.com/webinar/25530/critical-success-factors-for-sample-collection-storage-and-retrieval/

Leica Microsystems recently launched new versions of their LMD systems, the Leica Microsystems LMD6 and LMD7. The new stands offer a bigger field of view at the camera ports, plus the choice between Halogen and LED transmitted light. Combined with a fresh new design, the newest Leica Microsystems LMD systems are an exciting choice for high performance and uncompromised quality.

Learn about the advantages of using Leica Microsystems LMD techniques for precise, contamination-free isolation of specific cell types. Using brain or plant tissue sections as an example, this webinar will provide an overview of the scientific and practical considerations for obtaining highly pure material for further molecular analysis in the field of Parkinson’s disease and plants.

For more information visit: http://bitesizebio.com/webinar/25633/leica-microsystems-laser-microdissection-dissection-perfection/

Having worked in an uptight academic lab, a very laid-back Danish biotech company, a typically British biotech lab and been my own working-from-home boss for a number of years I have tried many different approaches and structures for my day, work and life.

Although it is still a work in progress, the result is that I have some concrete guidelines that I know make my work more enjoyable and productive. In this webinar I’d like to share them with you so you can try them out to see if they work for you too.

For more information visit https://www.youtube.com/watch?v=y3pEQqpyXIE&list=PLvFacfba2Wcdcl2efK31e47LXxqnMh-CG

For more information visit: http://bitesizebio.com/webinar/25629/getting-correct-color-from-your-camera/

For more information visit: http://bitesizebio.com/webinar/24339/the-best-of-both-worlds-combining-light-sheet-and-confocal-microscopy/

Practical advice for sample preparation, qPCR setup and result analyses Guidance on choosing the correct fluorescent labeling system for your qPCR Tips to troubleshoot the most commonly encountered issues in qPCR Join Dr. Karen O’Hanlon Cohrt for a practical tour of the process, applications and practice of qPCR, from start to finish. Beginning with an overview of how qPCR works, the webinar will give practical advice on choosing the right setup for a given experiment. You’ll also get essential advice for carrying out your experiment, including tips for sample preparation, PCR setup and analyses. Dr. O’Hanlon Cohrt will also suggest what you can do if things go wrong!

The webinar will also cover the main applications:

Identifying genes with altered expression in disease states, such as cancer Detecting disease-related gene expression and identifying microbes Viral genotyping and viral load determination Understanding biochemical and signaling pathways and other fundamental research Register now! Don’t miss this opportunity to brush up on your qPCR.

For more information visit: https://bitesizebio.com/webinar/qpcr-tips-workflow-applications-and-troubleshooting/

For more information visit: http://bitesizebio.com/webinar/25528/liquid-biopsy-sample-handling/

First introduced in August 2014, the Airyscan detector from ZEISS represents a new detector concept for laser scanning microscopy (LSM) that enables a simultaneous resolution and signal-to-noise (SNR) increase over traditional LSM imaging. The Airyscan detector design substitutes the conventional LSM detector and pinhole scheme for an array of 32 sensitive GaAsP detector elements, arranged in a compound eye fashion that resides in the pinhole-plane while still generating an optical section. The new detection geometry allows for the collection of the spatial distribution of light originating from every point of a microscopic fluorescent object at the pinhole allowing access to higher frequency information and while additionally collecting more light for ultra-efficient imaging. Based on the Airyscan detection concept, the next innovation from ZEISS has been developed with the introduction of the Fast mode for Airyscan. The Fast mode concept utilizes the Airyscan detector technology in combination with an illumination shaping approach to enhance acquisition speeds by four times while simultaneously increasing SNR and resolution overcoming the traditional compromises of confocal imaging.

For more information visit: https://bitesizebio.com/webinar/zeiss-lsm-800-with-airyscan-your-compact-confocal-power-pack/

Successful ChIP experiments demand chromatin that is sheared to manageable fragments sizes while retaining the integrity of the DNA, preserving protein epitopes and the formaldehyde cross-links attaching the proteins of interest to the DNA to deliver sensitive and reproducible results. Current methods for shearing chromatin to the desired size for immunoprecipitation in ChIP experiments are a source of significant imprecision, adversely affecting the reproducibility of ChIP experiments. In this webinar, scientist from Covaris will discuss how to better optimize your sample preparation to improve the reproducibility of your results and present data demonstrating how Covaris’ AFA Technology is ideally suited to provide the highest quality sheared chromatin. Shearing chromatin with AFA preserves precious epitopes, maintains DNA quality, protects from shearing biases, and provides highly reproducible results which are the reasons AFA is quickly becoming the standard chromatin shearing solution in leading epigenetic labs.

For more information visit: https://bitesizebio.com/webinar/optimizing-chip-for-reproducibility/